Application

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

ELISA Analysis: A representative lot of this antibody detected endogenous Apolipoprotein D in normal human plasma (Knipping, G., et al. Journal of Immunological Methods 202 1997. 85–95)
Western Blotting Analysis: A representative lot of this antibody at 1:5000 dilution detected endogenouse Apolipoprotein D in 5 µl of human plasma (Bottcher, A., et al. J. Lipid Res. 2000. 41: 905–915.)

Anti-Apolipoprotein D is an antibody against Apolipoprotein D for use in ELISA, WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Apolipoprotein D (ADOP), also known as Apolipoprotein D, Apo-D, ApoD, and encoded by the gene name APOD, is a member of the calycin superfamily and the Lipocalin family. Recent studies have suggested that Apolipoprotein D is involved in the transport and binding of bilin. Additioanlly, studies have demonstrated that Apolipoprotein D is able to transport a variety of ligands in a number of different contexts. Apolipoprotein D is a homodimer located in the plasma, and it also exists as a disulfide-linked heterodimer with APOA2. Apolipoprotein D is expressed in the liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. Apolipoprotein D is primarily localized in HDL (60-65%), with most of the remainder in VHDL and only small amounts in VLDL and LDL.

Immunogen

Protein purified from human plasma corresponding to Apolipoprotein D.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting on purified human Apolipoprotein D

Western Blotting Analysis: A 1:1000 dilution of this antibody detected 0.05 µg of purified human Apolipoprotein D

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~30 kDa observed, additional bands likely due to heavy N-glycosylation on this protein